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Journal: Frontiers in Cell and Developmental Biology
Article Title: Transcriptomic Analysis Identified ARHGAP Family as a Novel Biomarker Associated With Tumor-Promoting Immune Infiltration and Nanomechanical Characteristics in Bladder Cancer
doi: 10.3389/fcell.2021.657219
Figure Lengend Snippet: Knockdown of ARHGAP5-, ARHGAP17-, and ARHGAP24-suppressed BC cell proliferation in vivo and in vitro . (A) System analysis of expression of ARHGAP family gene in BCa cancer cell lines in the CCLE database. (B) ARHGAP5, ARHGAP17, and ARHGAP24 expression in seven pairs of low-grade BCa and seven pairs of high-grade BCa; GAPDH serves as a loading control. (C) ARHGAP5, ARHGAP17, and ARHGAP24 expression in four bladder transitional cell carcinoma cell lines (RT4, UM-UC-3, T24, and 5637) compared with immortalized epithelium cell line (SV-HUC-1). (D,E) Cloning formation of T24, UMUC-3, and ARHGAP5, ARHGAP17, and ARHGAP24 knocking down T24, UMUC-3 (*** p < 0.001 versus shNC). (F,G) 5-Ethynyl-2′-deoxyuridine (EdU) assay of T24 and shARHGAP5-, ARHGAP17-, and ARHGAP24-transfected T24 (*** p < 0.001 versus shNC). (H) Tumor growth size of subcutaneous T24 tumors transfected with shNC or shARHGAP5, ARHGAP17, and ARHGAP24 21 days after tumor implantation. (I) Tumor growth curve of subcutaneous T24 tumors transfected with shNC or shARHGAP5, ARHGAP17, and ARHGAP24 in 21 days of tumor implantation. (J) Average weights of tumors from shNC and shARHGAP5, ARHGAP17, and ARHGAP24 are shown in the histogram. Error bars indicate SD.
Article Snippet:
Techniques: Knockdown, In Vivo, In Vitro, Expressing, Control, Cloning, EdU Assay, Transfection, Tumor Implantation